The Effects of Dexmedetomidine on Injury Induced by Oxygen and Glucose Deprivation/Reperfusion in a Neuroblastoma Cell Line of Mouse Origin
Tülün Öztürk1, Ertan Darıverenli2, Elgin Türköz Uluer3, Tuna Önal3, Dilek Gülce Tatlı2, Kamil Vural21Department of Anesthesiology and Reanimation, Celal Bayar University Faculty of Medicine, Manisa, Türkiye2Department of Pharmacology, Celal Bayar University Faculty of Medicine, Manisa, Türkiye
3Department of Histology and Embriology, Manisa City Hospital, Manisa, Türkiye
INTRODUCTION: The aim of this study was to investigate the preliminary effects of different doses of dexmedetomidine (DEX) on cell viability and neurite growth, as well as the effects of pre- or post-treatment with DEX on oxygen and glucose deprivation/reoxygenation (OGD/R)-induced apoptosis and inflammation in mouse neuroblastoma (NB2a) cells.
METHODS: The effects of DEX on cell viability and toxicity were explored using the MTT and NNT methods at DEX concentrations of 0.1, 1, 3, 10, 30, 45, and 60 μM. To investigate the effects of DEX (10 μM) on OGD/R-induced apoptosis and inflammation, four experimental groups were established: Control group (C), in which cells did not undergo the OGD/R procedure; OGD/R group, in which cells were not treated with DEX; DEX+OGD/R group, in which cells were treated with DEX (10 μM) before the OGD/R procedure; and OGD/R+DEX group, in which cells were treated with DEX (10 μM) after the OGD/R procedure. Data were analyzed using one-way ANOVA (post hoc test: Tukey-b) and the Kruskal–Wallis test. Data were presented as mean±SD. P<0.05 was considered statistically significant.
RESULTS: Dexmedetomidine increased cell viability at all concentrations (0.1–45 μM) except 60 μM. H-scores for eNOS and TGFβ antibodies in the DEX+OGD/R and OGD/R+DEX groups were significantly decreased compared with the OGD/R group following treatment with DEX 10 μM (p<0.001). The apoptotic index in the DEX+OGD/R and OGD/R+DEX groups was also significantly decreased compared with the OGD/R group (p<0.001).
DISCUSSION AND CONCLUSION: Dexmedetomidine increased cell viability at almost all concentrations (0.1–45 μM) except 60 μM. Treatment with DEX 10 μM, either before or after injury, reduced apoptosis induced by OGD/R injury in NB2a cells. Pre-injury treatment with DEX also decreased both iNOS and eNOS antibody expression. The neuroprotective effects of DEX against OGD/R injury may be attributed to its antiapoptotic and antioxidative effects.
Keywords: Apopitosis, dexmedetomidine, IL-6, NB2a, OGD/R
Manuscript Language: English